Morphological and Molecular Identification of Colletotrichum gloeosporioides Causing Walnut Anthracnose in Shandong Province,China
Walnut anthracnose is a wide-spread and destructive disease in China.The aetiological agent was isolated from typical anthracnose lesions of infected fruits, leaves and shoots, and morphological characteristics and phylogenetic analysis based on ITS were used to identify the pathogen of walnut anthracnose.The result showed that colonies of these isolates could be divided into three types by conidia shape: cylindrical, with an average size of 10.4-15.6×5.4 pm; cylindrical, but occasionally slightly acute on the base, the average size of 11.7×4.6 μm; cylindrical, slightly curved middle or dumbbell shaped, the average size of 15.0×6.4 μm.The appressoria are dark brown or light brown, with ovoid, suborbicular, crack leafy and irregular form, the size is different with an average of 10.2×7.3 μm.Phylogenetic analysis using ITS showed that all isolates form a compact cluster with Colletotrichum gloeosporioides.Hence, based on morphological and molecular data, it could be concluded that C.gloeosporioides is the pathogen causing walnut anthracnose in Shandong province, China.
walnut diseases anthracnose etiology ITS sequences phylogenetics
Y.F.Zhu Y.F.Yin W.W.Qu K.Q.Yang
Forestry College and Key Laboratories Ecological and Environmental,Shandong Agricultural University, Forestry College,Shandong Agricultural University,Key Laboratory of Crop Biology,Taian,China
国际会议
The 7th International Walnut Symposium(第七届世界核桃大会)
山西汾阳
英文
353-359
2013-07-20（万方平台首次上网日期，不代表论文的发表时间）