The Effects of Visible Light Radiation(400-500 nm)on Enzymatic Activity of Collagenase
Debridement is the process of removing necrotic burden or contaminated tissue from a wound bed until surrounding healthy tissue is exposed.Enzymatic debridement helps to remove non-viable tissue which can otherwise delay wound healing and lead to infection 1.Collagenase enzyme is known to be able to promote cellular responses to injury and wound healing in vivo.Collagenase shows more selectively on denatured collagen in devitalized tissue.This selectivity is beneficial as it keeps the vital tissue and growth factors crucial to wound healing intact.There are studies which have shown that applied electromagnetic radiation(EMR)in the visible light range can modulate protein and cellular activity.Here we validate experimentally the hypothesis of the Resonant Recognition Model(RRM)that selectivity of protein activities is based on specific resonant electromagnetic interactions 2.The computational analysis of 28 collagenase sequences was performed and the activation frequency/wavelength range was determined to be 450-460 nm.To evaluate this range,the Collagenase enzyme solutions were irradiated by monochromatic light of 400 to 500nm.The kinetics of the chemical reaction was measured by continuous monitoring of the changes in absorbance of collagen at 570nm.The results revealed that collagenase activity can be modulated at the particular wavelengths of 450nm,456nm,and 460nm,which is within the activation wavelength range defined computationally.This finding indicates that enzyme function can be modified by an applied electromagnetic radiation of defied frequency,which may contribute to the development of a new clinical therapy for wound healing promotion.
J.Hu V.Vojisavljevic E.Pirogova
School of Electrical and Computer Engineering RMIT University,Melbourne 3001,Australia
国际会议
Progress in Electromagnetics Research Symposium 2013(2013年电磁学研究新进展学术研讨会)
台北
英文
392-396
2013-03-01（万方平台首次上网日期，不代表论文的发表时间）