Experimental Evaluation of Cytotoxicity Effects in Cancer and Normal Cells Exposed to Far Infrared Radiation
It has been proven that many of biological processes are frequency selective processes that relate to quantum energy state of photosensitive molecules.It was shown that light-activated changes in protein energy states can induce or modulate biological processes.Various up-to-date methodologies that incorporate low-intensity light into therapeutic procedures have been integrated into modern medicine.Here we have studied experimentally the hypothesis of the Resonant Recognition Model(RRM)that selectivity of protein activities is based on specific resonant electromagnetic interactions 1.The RRM theory proposes that an external electromagnetic field at a particular activation frequency would produce resonant effects on protein biological activity,and this activation frequency can be determined computationally 1.In our previous study 2 it was proposed that the wavelengths of the applied electromagnetic radiation(EMR)in a range of 3500-6400nm are expected to affect biological activity of oncogene and proto-oncogene proteins 2,3.Thus,in this study we designed an exposure system based on IR-LED to irradiate the selected cancer and normal cells in the wavelength range predicted computationally by the RRM.The experimental evaluation of the attained far infrared wavelengths of 3400nm,3600 nm,3800nm,3900nm,4100nm and 4300nm was conducted on a mouse melanoma(B16F0)and Chinese Hamster Ovary(CHO)cell lines.CHO cells are normal cells and used here as a control and B16F0 is cancer cell line.A comprehensive quantitative analysis of the exposed and sham-exposed B16F0 and CHO cells has been carried out.The results obtained from LDH cytotoxicity test of B16F0 and CHO cells exposed to the computationally predicted wavelengths of far IR light presented and discussed here.In addition qualitative analysis of the effects of applied radiation on cancer and normal cells was performed using the light microscopy.The significance of the findings obtained from the cytotoxicity effects measured by LDH test as well as light microscopys results is discussed and compared with the computational predictions.
P.Peidaee T.Istivan R.Shukla E.Pirogova
School of Electrical and Computer Engineering,RMIT University,Melbourne,Australia Department of Biotechnology and Environmental Biology,School of Applied Sciences RMIT University,Bun Department of Applied Chemistry,School of Applied Sciences RMIT University,Bundoora,Victoria,Austral School of Electrical and Computer Engineering,RMIT University,Melbourne,Australia;Health Innovation
国际会议
Progress in Electromagnetics Research Symposium 2013(2013年电磁学研究新进展学术研讨会)
台北
英文
919-922
2013-03-01（万方平台首次上网日期，不代表论文的发表时间）