A new Cytotoxicity Assessment Method using concentration & time cellular response curves
Many toxicity indices have been developed to assess the cytotoxicity,such as LC50,AUC50 and KC50 and so on.Comparing to the end-point indices(such as LC50),the dynamic indices(AUC50,KC50)can capture the kinematic information of cell growth inhibition or cell damage.However,those dynamic indices are very sensitive to the experimental noise and the shape of cellular response curve.In order to overcome those problems,a new toxicity index,named DC50 using the Fast Fourier transform(FFT)algorithm,was proposed in this study.The proposed index describes cellular kinematic behavior when cells exposed to the test chemical.Bio-experiment of HepG2 cell line exposure to 14 test chemicals was used to demonstrate theperformance of DC50.According to the analysis using the Register Cytotoxicity(RC)model,the correlation between DC50 and LD50 75.98%and the proposed cytotoxicity assessment method can be an alternative to the traditional cell based in-vitro assay.
Register Cytotoxicity (RC) DC50 Fast Fourier transform (FFT) HepG2
PU Tianqing PAN Tianhong
School of Electrical & Information Engineering,Jiangsu University,Zhenjiang,Jiangsu 212013
国际会议
The 33th Chinese Control Conference第33届中国控制会议
南京
英文
6868-6872
2014-07-28（万方平台首次上网日期，不代表论文的发表时间）