An evanescent wave planar waveguide biosensor for highly sensitive detection of bisphenol A in water sample
A compact and portable evanescent wave planar waveguide biosensor is developed for the highly sensitive detection of bisphenol A (BPA), which employs fluorescence-based detection of the binding of fluorophore-tagged antibodies to thesurface of a planar optical waveguide chip.The incident light is coupled into the planar optical waveguide chip via a beveled angle to form an individual reflection spot for the analyte biosensing.The fluorescence emission is collected by the multi-mode fiber placed in parallel underneath the chip.A reusable biosensing surface is established via the covalent attachment of the analyte derivative formed onto the optical waveguide chip.Typical calibration curves obtained for BPA has detection limits of 0.03 μg/L.Linear response for BPA ranged from 0.124-9.60 μg/L.The 50% inhibition concentration(IC50) for BPA were 1.09-±0.25 μg/L.The regeneration of the planar optical waveguide chip allows the performance of more than 300 assay cycles within an analysis time of about 20 min for each assay cycle.
Xu Wei-qi Liu Lan-hua Zhou Xiao-hong Shi Hant-chang He Miao
State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing, China
国际会议
北京
英文
62-63
2013-11-07（万方平台首次上网日期，不代表论文的发表时间）