A Novel Strategy for fast determination of Antibiotic Resistance Using Enzyme Specificity Fingerprinting
Antibiotic resistance (AR) is a major cause of medical treatment failure.The production of antibiotic degrading enzymes is the most common mechanism of AR.The carbapenems are the most effective antibiotics currently available due to their broad spectrum and resistance to hydrolysis by extended-spectrum β-lactamases.Recently β-lactamase variants have been isolated that can readily hydrolyze most β-lactam antibiotics, including carbapenems, carbapenemases.In addition, they were not inhibited by therapeutic β-lactamase inactivators.Infections by carbapenemase producing Enterobacteriaceae are life threatening with mortality rates ranging from 20% to 40%.Carbapenemases have been identified in all four (A, B, C, and D) classes of β-lactamases.The recent isolation of large AR plasmids containing multiple resistance genes, rise in horizontal transmission and increasing AR persistence are further indications that we are losing the battle to control AR.With few new antibiotics against Gram-negative bacteria under advanced development, there is an urgent need to find ways to slow the spread of AR.
Bin Xie
Department of Pure and Applied Biochemistry, Lund University Sweden
国际会议
北京
英文
64-65
2013-11-07（万方平台首次上网日期，不代表论文的发表时间）