Optimization of Bmlebocin3 Expression using Design of Experiment

摘要：

　　DOE (design of experiment) was verified as an effective way to increase productivity and improve quality in physical,materials,social sciences and agricultural engineering.For protein expression in molecular biology optimization is very necessary to get tiie maximum recombinant protein.Traditionally,it usually takes factors in consideration separately,therefore,tiie relationships between factors are ignored.In this study,DOE was applied to optimization of Bmlebocin3 precursor expression,and tiie results show tiiat besides OD value when induction begins and time of induction tiiese two factors,the relationship between these two factors can significantly influence the protein expression of Bmlebocin3.

关键词： DOE Optimization Protein expression Bmlebocin3

作者: Zhi Li Yi Li Xuan Liu Xi-Qian Lan Polly Hang-Mei Leung Jia-Shen Li Frank Ko Ling Qin

作者单位: Institute of Textiles and Clothing,The Hong Kong Polytechnic University,Hung Hom,Kowloon,Hong Kong Institute of Sericulture and Systems Biology,Southwest University,Chongqing,400716,China Department of Health Technology and Informatics,The Hong Kong Polytechnic University,Hung Hom,Kowloo Department of Materials Engineering,The University of British Columbia,Canada Department of Orthopaedics & Traumatology,The Chinese University of Hong Kong,Hong Kong,China

会议类型: 国际会议

会议名称: 第六届纺织生物工程及信息国际会议(The 6th Textile Bioengineering and Informatics Symposium )(TBIS2013)

会议地点: 西安

会议语种:英文

页码: 230-239

在线出版日期: 2013-09-26（万方平台首次上网日期，不代表论文的发表时间）