Differential Expression of Stage-specific Fat Body Proteins during Larval-pupal Period in Silkworm(Bombyx mori)
The silkworm fat body is the organ of many intermediary metabolic processes and sustenance of growth throughout the life, and its proteins are responsible for storing nutrients, providing energy and regulating hormones.To date, detailed differential expressions of fat body proteins have not been clearly analyzed.In this research, fat body proteins were extracted from the larvae of the 5th day of the 5th instar, un-pupated larvae of spinning end and pupae of pupating day of the multivoltine silkworm variety Da Zao, and the differential expressed proteins were characterized by two-dimensional gel electrophoresis, followed by mass spectrometry identification and informatics method.14 stage-specific expression proteins were detected and confirmed.Among them, 7 proteins were accurately identified, such as actin, calponin-like protein, beta-tubulin, hypothetical protein, antichymotrypsin precursor and 30K protein precursor etc.The biological roles of these important proteins during the larval-pupal developmental stages were discussed.
silkworm fat body protein differential expression stage-specific mass spectrometry two-dimensional electrophoresis (2-DE)
Chi Chen Yan-yan Liu Zhi-ping Wu Rui-he Niu Jian-zhong Tan
School of Architecture and Urban Environment,Soochow University,Suzhou,P.R..China Jiangsu Huajia Silk Co.,LTD,Shengze Town,Wujiang District,Suzhou,P.R.China
国际会议
苏州
英文
67-71
2013-09-09（万方平台首次上网日期，不代表论文的发表时间）