Characteristics of ammonia-oxidizing archaea in the hyporheic zone of Fuyang River, China
Nitrification plays a significant role in the global nitrogen cycle. It has been widely accepted that ammonia-oxidizing bacteria (AOB) are responsible for nitrification. However, this concept is currently challenged by the discovery and ubiquity of ammonia-oxidizing archaea (AOA) in various environments, such as in marine and terrestrial ecosystems. In this study, the presence and distribution of AOA in the hyporheic zone of Fuyang River along the vertical nutrient gradient was documented via archaeal amoA amplification; the diversity was investigated by clone libraries, sequencing and phylogenetic analysis; the abundance was estimated by real-time polymerase chain reaction (qPCR). The archaeal amoA gene was detected in different layers of the sedimentary samples of the hyporheic zone and the archaeal communities varied little with depth. Based on retrieved sequences and phylogenetic analysis, the sequences were affiliated within the marine Group 1.1a and soil Group 1.1b of Group 1 Crenarchaeota, dominantly by Nitrosopumilus-like and Nitrososphaera-like AOA, respectively. The quantification results revealed that the copy number was in the range of 1.48 ×107 to 6.18 ×109 copies per gram of dry sediment. Furthermore, it was found that the composition of community was mainly affected by total nitrogen, total organic carbon and ammonia. This study provides a vital supplement to the distribution of AOA in global pattern, and offers implications for further understanding of the potential role of archaeal oxidizers in river hyporheic zone ecosystems.
ammonia-oxidizing archaea hyporheic zone diversity abundance Fuyang River
Z.X.Wang Z.Y.Wang Y.S.Pei
The Key Laboratory of Water and Sediment Sciences, Ministry of Education, School of Environment, Beijing Normal University, No. 19, Xinjiekouwai Street, Beijing 100875, PR China
国际会议
The 3rd Biennial ISRS Symposium Achieving Healthy and Viable Rivers (ISRS)第3届国际河流大会
北京
英文
610-620
2013-08-05（万方平台首次上网日期，不代表论文的发表时间）