Analysis of Apoptosis Mechanism in SGC-7901 Induced by CSBE with Flow Cytometry and Confocal Laser Scanning Techenology
Flow cytometry is a laser based,biophysical technology employed in cell counting,sorting,biomarker detection and protein engineering,by suspending cells in a stream of fluid and passing them by an electronic detection apparatus.Confocal laser scanning microscopy is a technique for obtaining high-resolution optical images with depth selectivity.The flow cytometry and confocal laser scanning microscopy were used to study the changes of initiator,effector and marker in process of mitochondrial apoptosis pathway induced by CSBE.In addition,the regulation genes of apoptosis were surveyed with western blotting and flow cytometry.As a result,the mechanism of apoptosis induced by CSBE was to increase the expression of pro-apoptotic protein Bax,decrease the expression of antiapoptotic protein Bcl-2,which induced mitochondrial PTP channel open,decreased mitochondrial membrane potential,led to release of mitochondrial Cyt-c and caused activation of caspase-3.To sum up,the mitochondrial apoptosis pathway of SGC-7901 induced by CSBE was revealed by the flow cytometry and confocal laser scanning microscopy.
Apoptosis mechanism SGC-7901 CSBE Flow cytometry Confocal laser scanning techenology
Lei Yu Yubin Ji Miao Yu Shiyong Gao Xiang Zou Chenfeng Ji
Center of Research and Development on Life Sciences and Environmental Sciences Engineering Reseach C Engineering Reseach Center of Natural Anticancer Drugs Ministry of Education Harbin 150076, China
国际会议
2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)
北京
英文
54-58
2013-05-25（万方平台首次上网日期，不代表论文的发表时间）