Radiation Enhance Biological Activity of Recombinant Adeno-associated virus against Human Glioma through Down-regulating ATM

摘要：

　　Object Whether radiation can improve the biological activity of recombinant adeno-associated virus (rAAV) in U251 cells and the molecule mechanism.Methods 60Co as the radiation resource to irradiate tumor cells; Inverted fluorescence microscope was performed to observe expression of EGFP; flow cytometry (FACS) analysis for transduction efficiency and mean fluorescent intensity; RT-PCR analysis for mRNA expression of rAAV binding receptors genes—HSPG,FGFR1and Integrin,and DNA circularization related gene—ATM.Results Transduction efficiency don’t have a notable change after radiation.But the mean fluorescent intensity was improved with the rise of radiation dose.In mRNA expression level,the binding receptors for rAAV targeting cells,as well as ATM,have down-regulated.Conclusion Radiation can enhance biological activity of rAAV,and the enhance effect is not related to the “rAAV binding receptors procedure.But down-regulation of ATM might affect this process.

关键词： radiation recombinant adeno-associated virus biological activity ATM

作者: Zuye Yang Yunjia Zhang Zhaoying Fu Hailong Wang Hong Ma

作者单位: School of Life Science, Beijing Institute of Technology, Beijing 100081, China College of Life Science, Yan’an University, Yan’an 716000, China

会议类型: 国际会议

会议名称: 2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 165-168

在线出版日期: 2013-05-25（万方平台首次上网日期，不代表论文的发表时间）