Fast Neutron Irradiation Induces Regionally Specific Activation of TRAIL/DR5 Pathway in Rat Brain
Object To explore the effects of neutron-irradiation exposure on TRAIL/DR5 pathway in rat brain regions.Methods Fast neutronirradiation was employed as the irradiated model.The expression changes of TRAIL and DR5 in cerebral cortex,hippocampus,cerebellum and corpus striatum were detected after 1,7 and 14 d post-irradiation by RT-PCR,ELISA and western blotting.Cleaved caspase-8 was examined by western blotting.Results In protein expression level,TRAIL was increased in the four regions to different extents after 7 d and then recovered after 14 d; DR5 was detected to be up-regulated in cerebral cortex,hippocampus and corpus striatum but down-regulated in cerebellum after 14 d postirradiation.No significant changes of TRAIL and DR5 were observed in RNA level by RT-PCR.The cleaved caspase-8 was up-expressed the greatest after 1 d in cerebral cortex,hippocampus and cerebellum but continually up-regulated in corpus striatum from 1 to 14 d after irradiation treatment.Conclusion TRAIL was induced in rat brain at early stage while the expression changes of DR5 were delayed by neutron-irradiation and vary in different brain regions.Moreover,caspase-8 was not activated in accord with the changes of TRAIL and DR5 in some brain regions.These indicate that the activation of TRAIL/DR5 pathway plays a role in irradiation-induced injury,which is regulated in a region-specific and time-dependent manner.
Fast neutron-irradiation TRAIL/DR5 pathway Rat brain regions
Ling Rao Hailong Wang Zhengang Shan Jieying Zheng Xiao Wang Hong Ma
School of Life Science, Beijing Institute of Technology, Beijing 100081, China China Institute of Atomic Energy, Beijing 102413, China
国际会议
2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)
北京
英文
215-218
2013-05-25（万方平台首次上网日期，不代表论文的发表时间）