The formation of G-quadruplex in biologically relevant DNA sequences induced by alkaloid

摘要：

　　Many Guanine-rich DNA sequences are capable of forming G-quadruplex,such as the telomeric chromosomal terminals and the promoters of several genes including c-myc,ckit and bcl-2 oncogenes.G-quadruplex structure have conspicuous biological significance and hence the ligands which can promote the formation and stabilize G-quadruplex have attracted much attention.In this paper,we have evaluated the abilities of Jatrorrhizine and Chelerythrine in inducing the formation of G-quadruplex employing Circular Dichroism spectroscopy.Our results indicate that Jatrorrhizine can induce Pu18 and c-kit87up to form mixed-type and parallel Gquadruplex respectively.Chelerythrine can induce Pu18 and ckit87up to form parallel and antiparallel G-quadruplex respectively.Jatrorrhizine and Chelerythrine can induce the switch of Pu18 G-quadruplex in the presence of K+ from mixedtype to antiparallel and parallel respectively.However,Jatrorrhizine and Chelerythrine can not change the conformation of c-kit87up G-quadruplex in the presence of K+.From experimental results it is clear that Jatrorrhizine and Chelerythrine may have the potential to inhibit the growth of tumour cell and also can be useful guidelines for the design of anticancer agents.

关键词： G-quadruplex oncogene alkaloid induce

作者: Run Hao Yameng Liu Rugang Zhong

作者单位: Department of Life Science and Bioengineering Beijing University of Technology Beijing, China

会议类型: 国际会议

会议名称: 2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 260-263

在线出版日期: 2013-05-25（万方平台首次上网日期，不代表论文的发表时间）