A Mechanical Perforation Procedure for Embryo Biopsy

摘要：

　　This paper presents an active mechanical perforation method developed for embryo biopsy in the preimplantation genetic diagnosis (PGD) procedure.This mechanical approach utilizes a tungsten micro-needle with a sub-micrometer tip to perform embryo perforation.The micro-needle is driven longitudinally with sinusoidal or square wave signals at frequencies close to the micro-needle bending resonances in order to excite its lateral vibration modes due to nonlinear modal coupling.It has been demonstrated that,in particular,the lateral cutting method,is effective and suitable for embryo perforation as well as microinjection and micro-dissection.A micro-needle driven with a square wave signal at frequency (850Hz) close to the micro-needle first bending resonance frequency has been shown to generate an accurate cutting dimensions with minimum damage to the mouse embryo zona pellucida.A success rate of 88% was achieved based on eight perforation tasks.

关键词： Microsurgery PGD embryo biopsy perforation

作者: Issam M. Bahadur James K. Mills

作者单位: Department of Mechanical and Industrial Engineering University of Toronto Toronto, Ontario, Canada M5S 3G8

会议类型: 国际会议

会议名称: 2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 313-318

在线出版日期: 2013-05-25（万方平台首次上网日期，不代表论文的发表时间）