Determination Human Brain Tumor Marker Gene Carbonic Anhydrase 9 (CA9) Gene Expression in Different type of Brain Tumor Cells
The hypoxia-inducible enzyme carbonic anhydrase IX (CA-IX) is considered as a surrogate marker of tumor hypoxia,prognosis indicator and a potential the expression pattern of the human tumor hypoxia marker gene CA-IX protein,CA9-mRNA and hypoxia-inducible factor-1α (HIF-1α) protein levels in GaMG cells exposed to in vitro hypoxia (1,6 or 24 h at 5%,1% or 0.1% O2).All cell lines displayed a strong hypoxic induction of CA9-mRNA in response to prolonged severe hypoxia with cell-line specific patterns at moderate to mild hypoxia and shorter treatment times.In GaMG cell lines,a marked induction of CA-IX protein in response to severe hypoxia was seen.CA-IX over expression was paralleled by the results for HIF-1α protein.CA-IX expression pattern in human malignant glioblastoma cells (GBM) resulted from low oxygen concentrations or constitutive,oncogene-related,over expression is considered prognostically relevant.
Human Brain Tumor Marker Materials and Methods Results Discussion and Conclusions
Harun M. Said
Dept. of Radiation Oncology University of Würzburg Josef Schneider Str. 11, 97080 Würzburg, Germany
国际会议
2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)
北京
英文
403-407
2013-05-25（万方平台首次上网日期，不代表论文的发表时间）