Immobilization of BSA on The Polymethacrylic Acid

摘要：

　　N,N-Methylenebisacrylamide was used as the crosslinking agent to prepare the polymethacrylic acid and its carboxyl groups were modified by N- (3- Dimethy- -laminopropyl)-N’ –ethyl carbodiimide hydrochloride and N-Hydroxy succinimide.The ability of immobilizing BSA and the influence of reacting time of carrier were studied.The immobilization amount and the optimal reacting time were 2100mg/g and 90 minutes respectively.The carrier was characterized by infrared detection.The results indicated that the polymethacrylicacid possessed good performance and the ability to immobilized BSA was enhanced after the modification of EDC-NHS.

关键词： polymethacrylicacid immobilized protein modified carrier

作者: Fang Lv Xiu-jie Liu Kai-yue Chi Rong-ji Dai Yu-lin Deng

作者单位: School of Life Science, Beijing Institute of Technology, Beijing 100081, china

会议类型: 国际会议

会议名称: 2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 501-503

在线出版日期: 2013-05-25（万方平台首次上网日期，不代表论文的发表时间）