The effects of granulosa cell apoptosis on the developmental competence of bovine oocytes:A study using the well-in-well culture system

摘要：

　　In the present study,we succeed to establish the well in the well (WIW) oocyte/embryo culture system.Under this system we studied the effect of granulosa cell apoptosis on oocyte developmental competence.The results show that the hatching rates of blastocysts did not differ (P > 0.05) between oocytes cultured in the WIW system (13%) and those cultured in the group system (16%).In 4-8mm follicles,oocyte developmental competence is higher (P < 0.05) in early atretic follicles (percentage of apoptosis 10-40%) than in the non-atretic follicles (percentage of apoptosis <10%) or in the late atretic follicles (percentage of apoptosis >40%).But in 2-3mm follicles,even though in early atretic follicles the developmental competence of oocyte is also poor (P>0.05).It was indicated that the granulosa cell apoptosis affected the oocyte developmental competence significantly.

关键词： oocyte developmental competence granulosa cell apoptosis

作者: Weiguo Feng Guohui Wang Zhifang Pan Chune Ren

作者单位: College of Pharmacy and Biological Science Weifang Medical University Weifang, Shandong province, Ch Reproductive Medical Center Affiliated Hospital of Weifang Medical University Weifang, Shandong prov

会议类型: 国际会议

会议名称: 2013 ICME International Conference on Complex Medical Engineering(2013 ICME复合医学工程国际会议)

会议地点: 北京

会议语种:英文

页码: 504-507

在线出版日期: 2013-05-25（万方平台首次上网日期，不代表论文的发表时间）