Lignosus rhinocerotis (Cooke) Ryvarden Induces Neuritogenesis via Extracellular Signal-Regulated Kinase (ERK) Pathway in Rat Pheochromocytoma Cells
Neuritogenic activity has become one of the focuses of study in search of preventive and therapeutic agents for neurodegenerative diseases.As one factor in the neurotrophin family,nerve growth factor (NGF) has potent biological activities,e.g.Promoting neuronal survival,proliferation,neuritogenesisand it is expected to be applied to the treatment of neurodegenerative diseases.Among the natural sources explored for NGF mimics,the potential of culinary and medicinal mushrooms has attracted intense interest.Traditional application of the sclerotium of Lignosus rhinocerotis by the indigenous folks as remedy and tonic to treat variety of ailments has beendocumented in Malaysia.This study aimed to investigate the potential of NGF-like activity ofL.rhinocerotis which may contribute to the prevention and treatment of the neurodegenerative diseases.The neuroendocrine cell line,rat pheochromocytoma (PC-12),was used as an in vitro model in this study.Nerve growth factor (50 ng/ml) was used as a positive control.The aqueous extract ofL.rhinocerotis sclerotium promoted neuritogenesisin PC-12 cells significantly (p<0.05) at a low concentration (25 tg/ml).The involvement of the mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MEK/ERK1/2) signaling pathway in aqueous extract-induced neuritogenesis was investigated using two specific inhibitors of the MEK/ERK1/2 pathway (U0126 and PD98059).The neuritogenic activity of aqueous extract was blocked by these inhibitors significantly (p<0.001).Furthermore,the protein expression of total p44/42 MAPK (Erk1/2) and phosphor-p44/42 MAPK was determined.The data revealed the first evidence of the involvement of MEK/ERK1/2 signalling pathway in aqueous extract of L.rhinocerotis sclerotium-inducedneuritogenesis in PC-12 cells.
Lignosus rhinocerotis Nerve growth factor MEK/ERK1/2 signaling pathway Neuritogenesis Neurodegenerative diseases
SYNTYCHELING-SING SEOW MURALI NAIDU PAMELA DAVID KAH-HUI WONG VIKINESWARY SABARATNAM
Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science; Institute of Biologi Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science;Department of Anatomy
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
177-180
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）