Cytotoxicity Effect of Selected Medicinal Mushrooms on Bv2 Microglial Cells
Microglialcells are the resident macrophages of the brain and spinal cord,and thus act as the first and main line of active defence in the central nervous system against assaults including inflammation.In the search for safe anti-inflammatory agents,mushrooms are being actively sought.This study was conducted to determine the cytotoxic effects ofvarious solvent extracts of three culinary and medicinal mushrooms-Lignosus rhinocerotis (Cooke) Ryvarden,Cordyceps militaris (L.) Link and Hericium erinaceus (Bull.:Fr.) Persto BV2 microglial cells.Cytotoxic effects were assessed by MTTassay.All fractions tested were not toxic to the BV2 cells at concentrations tested.Ethanol extracts(100%-191.77%) showed 35%-90%higher proliferation of BV2 cells when compared to hot aqueous extracts (19.38%-98.22%).The extract concentrations tested were 0.1ug/ml to 1000ug/ml.The ethanol extracts were further fractionated to hexane,ethyl acetate and aqueous fraction.These fractions were also tested for cytotoxic effects to the BV2 cells.The hexane fractions of the three mushrooms tested showed the highest proliferation (ranging 100%-200.16%) effects on the microglial cell line,followed byethyl acetate fraction (92.06 %-167.41%).However,the aqueous fraction (2.00%-93.21%) showed toxic effects to the cells in a dose dependent manner.
microglial cells medicinal mushrooms MTT culinary mushrooms solvent fractions
NEERANJINI NALLATHAMBY SRI NURESTRIABD.MALEK MURALI NAIDU WONG KAH-HUI PAMELA DAVID VIKINEWARY SABARATNAM
Mushroom Research Centre & Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpu Mushroom Research Centre & Department of Anatomy ,University of Malaya, 50603 Kuala Lumpur,Malaysia
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
234-238
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）