Comparison of The Anti-Tumor Activity of Polysaccharides Extracted by Boiling Water and Enzyme Assistance from Ganoderma lucidum

摘要：

　　Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum.In this work,polysaccarides were extracted from Ganoderma lucidum with boiling water method and enzyme assistant method.The human liver hepatocellular carcinoma cell line HepG2 was used to compare the anti-tumor effect of the two kinds of extraction with MTT test.Both of these two kinds of Ganoderma lucidum polysaccarides reduced cell viability of cancer cell HepG2 in a dose-and time-dependent manner.At low concentrations,there was no significant difference in the effectiveness of L1 and L2,while at concentrations over 0.8μg/mL the difference in the effectiveness of L2 in comparison to L1 became significant.At the concentrations of 3.2μg/mL,the cancer cells were almost killed in two days.

关键词： anti-tumor Ganoderma lucidum enzyme assistant extraction polysaccharides

作者: CHUNHUA XU CHENJU ZHANG ZHENLE TIAN HUIHUA ZHENG XIAOBINGYU

作者单位: Jiangsu Alphay Biological Technology Co., Ltd.No.68-A, Zhongyang Rd, Nantong city, Jiangsu Province,226009,China

会议类型: 国际会议

会议名称: The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)

会议地点: 北京

会议语种:英文

页码: 357-361

在线出版日期: 2013-08-26（万方平台首次上网日期，不代表论文的发表时间）