Pleurotus eryngii,also known as thistle mus~oom,is rich in proteins,dietary fiber and polysaccharides.It has been developed as a functional food due to its antioxidant,anti-fatigue,anti-viral and anti-tumor functions.Therefore,this study was conducted to investigate the immunoregulatory mechanisms and antioxidant effects of P.eryngii superfine powder (PESP) in vivo.Test mice were fed on a diet containing 6.67% PESP to explore its effect on their delayed type hypersensitivity (DTH).Subsequently,phagocytic index,serum hemolysin,serum and liver glutathione peroxidase (GSH-Px) activities,serum superoxide dismutase (SOD) activity,and malondialdehyde (MDA) content in liver homogenate were determined.For the human subjects,volunteers received PESP at doses of 5,10 or 15 g.Serum levels of 39 cytokines were assayed before and after ingestion using Millipore Human Kit and liquid chip scanner.Based on experimental results,dietary P.eryngii supplementation in mice significantly increased the weight difference of ears (p < 0.01),phagocytic index (p < 0.05),serum hemolysin (p < 0.01),serum and liver GSH-PX activities (p < 0.01),and serum SOD activity (p < 0.01),but decreased MDA content (p < 0.01) in liver homogenate.In human trials,5 g of PESP significantly decreased serum EGF,while 15 g of PESP significantly decreased serum granulocyte-macrophage colony stimulating factor (GM-CSF),macrophage-derived chemokine (MDC),and tumor necrosis factor-α (TNF-α).No change was detected in serum cytokines after the administration of 10 g of PESP.Thus,P.eryngii can regulate innate and acquired immune functions in mice to execute antioxidant and anti-aging functions,and can alter human serum cytokines,according to the results generated by this study.Moreover,the dual immunoregulatory effects ofPleurotus eryngii are consistent with the theory of traditional Chinese medicine.
Pleurotus eryngii immunoregulatory cytokine cell-cell communication network
QIUHUI HU YONG SUN YONGZHENGMA ZENGHUI XU WENJIAN YANG ALFRED MUGAMBI MARIGA GUANGCHANG PANG CHAOYU GENG
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Coll College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China Beijing Food Research Institute, Beijing 100162, China College of Food Science and Engineering, Nanjing University of Finance & Economics, Nanjing 210046, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;Depar College of Biotechnology and Food Science, Tianjin University of Commerce, Tianiin 300134, China