Separation and Purification of Ganoderic Acids by Macroporous Adsorption Resin and High Speed Countercurrent Chromatography
Objective Establish a new method of separating and purifying of Ganoderic acids from the extracts of Ganoderma lucidum with macroporous adsorption resin and high speed countercurrent chromatography (HSCCC).Methods A series of macroporous adsorption resins were compared on their adsorption and desorption properties for Ganoderic acids and the optimal type resin had been selected,the best ethanol concentration of sample and the ethanol gradients were determined by the relative technical parameters optimized; conducting a comparative analysis of partition coefficient K of Ganoderic acids in different solvent systems,and then the optimal solvent systems of HSCCC was determined.The partition coefficient (K) and the Ganoderic acids from HSCCC separation were measured by high performance liquid chromatography (HPLC).Results (1) HZ-816 was the most appropriate in separating and purifying of Ganoderic acids among the four macroporous adsorption resins,50% ethanol concentration was the optimum sample concentration,60%~80% for the ethanol gradients,and Ganoderic acids were eluted after 60% ethanol concentration.(2) The optimal solvent system was composed of petroleum ether-ethyl acetate-methanol-water (5:5:3:7,0.3% ethylic acid,v/v),the upper phase (petroleum ether-ethyl acetate) was used as stationary phase,the lower phase (methanol-water) as mobile phase,the flow rate of the mobile phase was 3mL/min,the rotation speed was 900 r/min,the detective UV wavelength was 254nm and the sample size was 20mL.Then two main components of Ganoderic acids were obtained by HSCCC separation,the purity of Ganoderic acid F was over 92% and the purity of Ganoderic acid C 1 was over 90% relatively; When the optimal solvent system was petroleum ether-ethyl acetate-methanol-water (5:5:1:9,0.3% ethylic acid,v/v),two Ganoderic acids were obtained that the purity of Ganoderic acid A was over 88% and the purity of Lucideric acid E2 was over 85% aiter HSCCC separation.Conclusion In this paper,the method of separating and purifying of Ganoderic acids with macroporous adsorption resin and HSCCC was adopted for the first time,it will not only save the test cost but also eliminate the environment pollution that cause by organic solvents with macroporous adsorption resin instead of the traditional silica gel column chromatography.Consequently,it will be conducive to the industrialized production of Ganoderic acids for the advantages including quickly,high sample capacity,high separation efficiency and so on of HSCCC.
Ganoderic acids macroporous adsorption resin high speed countercurrent chromatography
ZHU ZHONGMIN LI YE HUAZHENGGEN YAO WEIXI CHEN RUOYUN
Fujian Xianzhilou Biological Science & Technology Co., Ltd., National R&D Branch Center For Edible Fungi Processing, Fuzhou, 350002, China;Institute of Materia Medica, Chinese Academy of Medical Sciences,Beijing 100050
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
570-576
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）