Optimization of Enzyme Assisted Extraction of Polysaccharides from Ganoderma lucidum

摘要：

　　In this work,an enzyme assistant extraction method had been used to isolate rude polysaccaides form Ganoderma lucidum.The isolating effect optimized with orthographic graph statistic method with three level and four independent variables.Complex enzyme,extraction temperature,extraction time and extraction pH were combined to obtain the best possible combination to get maximum amount of extract and rude polysaccaides yield.The optimum extraction conditions were:complex enzyme amount of 3% (w/v),extraction temperature of 45 ℃,extraction time of 3 h and extraction pH of 7.Under these conditions,the experimental amount of extract was 8.9 % and the yield of rude polysaccharides was 1.1%,which were in close agreement with the value predicted by the model.

关键词： Ganoderma lucidum enzyme assistant extraction polysaccharides

作者: CHUNHUAXU, ZHENLE TIAN CHENJU ZHANG XIAOBING YU HUIHUA ZHENG

作者单位: Jiangsu Alphay Biological Technology Co., Ltd.No.68-A, Zhongyang Rd, Nantong city, Jiangsu Province,226009,China

会议类型: 国际会议

会议名称: The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)

会议地点: 北京

会议语种:英文

页码: 610-615

在线出版日期: 2013-08-26（万方平台首次上网日期，不代表论文的发表时间）