Mycelial Fermentation and Medicinal Properties of a Valuable Medicinal Fungus Ophiocordyceps sinensis Cs-Hk1
Ophiocordyceps sinensis (Cs) or DongChongXiaCao in Chinese is a rare and precious medicinal mushroom.In this work,a Tolypocladium sp.fungus named Cs-HK1 was isolated from a natural Cs fruiting body,and its UV,IR and HPLC spectra all exhibited high degrees of matching to those of natural Cs,indicating the similarity of chemical composition.A crude polysaccharide (PS) fraction isolated from Cs-HK1 fungal mycelium showed significant antitumor activity,inhibiting melanoma tumor growth in mice.The PS-treated mice showed significantly higher serum IFN-γ level and higher proliferation rate of lymphocytes ex vivo than the control animals,suggestive of the immunomodulatory activity of the PS fraction.In cell cultures,the PS fraction caused a significant and dose-dependent inhibitory effect on the proliferation of two cancer cell lines,B16/neu mouse melanoma and MDA-MB-231 human breast cancer cells,but not on normal spleen lymphocytes in vitro.Most of these bioactivities of the Cs-HK1 fungus were comparable to or even greater than those of natural Cs.The results show that Cs-HK1 fungus may be a functional substitute for the natural Cs in health food and herbal medicine.
Cordyceps sinensis Tolypocladium sp. Mycelia culture Antitumor Polysaccharide
JIANYONG WU
Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
655-665
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）