Optimization of Solid-State Fermentation Conditions for Fruiting Body Growthand Cordycepin Production by Cordyceps militaris
Cordyceps militaris (L.) Link has been recently used as functional food and medicine in China.In comparison with O.sinensis,C militaris contains similar biochemical components but a greater quantity of anti-cancer component cordycepin.For its significant medicinal effect,the market demand of artificial C.militaris has been continuously increasing.This study aimed to optimize the conditions of solid-state fermentation for fruiting body growth and cordycepin production from C.militaris by one-factor-at-a-time and orthogonal layout methods.The optimal culture substrate was brown rice.The optimal fruiting body growth and cordycepin production were observed at relatively low pH value.The optimum composition were 40 g/L glucose,5 g/L peptone,1.5 g/L MgSO4·7H2O,1.5 g/L K2HPO4 and 1.0 mg/L NAA for optimal fruiting body growth and 10 g/L glucose,10 g/L peptone,1.0 g/L MgSO4·7H2O,1.0 g/L K2HPO4 and 1.0 mg/L NAA for cordycepin production.These optimization strategies in solid medium culture lead to a 67.96 % (1.73±0.08 g/bottle) increase in fruiting body yield and a 63.17% (9.17±0.09 mg/g) increase of cordycepin yield in fruiting body,which may be applied in industrial production of cordycepin via solid-state fermentation.
Cordyceps militaris solid-state fermentation fruiting-body cordycepin optimization orthogonal
TINGCHI WEN GUANGRONG Li JICHUAN KANG CHAO KANG KEVIN D.HYDE
Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizho School of Life Science, Guizhou University, Guiyang 550025, Guizhou Province, China Institute of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
666-679
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）