Effect of Dietary Supplementation on Improvement of Growth and Immune Function of Broilers
The purpose of this investigation was to evaluate the effect of a dietary supplement (MirigenrM),a fungal cell wall derivative product,as a new generation alternative to antibiotics,on the growth and the innate and adaptive immune functions in broilers from birth to 45 days of age.Newbom chicken were randomly assigned to one of three groups (G):G1 controls (n=150) no supplement fed,G2 (n=150) fed with dietary supplement at a designed regular dose (0.5% w/w,weight of additive to food),G3 (n=150) fed with dietary supplement at double dose (1.0% w/w).All three groups were housed in the same conditions.Body weights (BW),and blood were taken on Day 1,14,28,and 45.Medications used and costs/treatment were recorded for each group.The whole blood was used to purify heterophils for reactive oxygen species (ROS) generation and E.Coli killing abilities examination assays,and the serum samples were preserved in freezer for ELISA assays to determine concentration of MIP-1β,CD4:CD8,IFN-γ,and titers of antibody against Newcastle disease virus (NDV).Group differences were analyzed using ANOVA algorithm (S-Plus).There was no significant birth weight difference in three groups.After 45 days growth,the dietary supplement treated groups had significant higher body weight gain with lower mortality rate if compared to the untreated control group (p <0.05).Their body weight gain and mortality rate are 2.23kg and 10% in G1 (control group),2.89kg and 2% in G2 (Experimental group,0.5% dose),and 2.77kg and 1% in G3 (Experimental group,1% dose),respectively.Heterophil ROS generation in treated groups were markedly improved through the addition of dietary supplement in both regular and double doses to the diet (p <0.05).The ability of heterophil to kill E.Coli was also significantly improved in dietary supplement treated groups(p <0.01).Comparing to control,there was significant higher IFN-γconcentration in treated groups (p <0.05) at day 45.The CD4/CD8 ratio was also improved in treated group (p <0.05).New castle disease is the most prevalent avian disease,and vaccination is effective method to protect the animals from the virus infection.In our study,it is found G2 and G3 groups that fed with dietary supplement had higher antibody titers against NDV after vaccination (P<0.01) and the antibodies lasted longer.Results from this study demonstrated dietary supplement to broilers improved the immune capabilities of immune cells,which are vital to the establishment of immune response against pathogens,thereby,to improve chickens health and growth and reduce medication cost in chicken farming.
broilers growth health Immunity Dietary Supplement
LIANGCHENG CHEN WEI ZENG YU LI YONGQIANG WANG
Mirigen Biotechnology Co.Ltd., Wuxi, Jiangsu Province 214174, PR China; Engineering Research Center Mirigen Biotechnology Co.Ltd., Wuxi, Jiangsu Province 214174, PR China Engineering Research Center of Edible and Medicinal Fungi, Jilin Agriculture University, Changchun,
国际会议
The 7th International Medicinal Mushroom Congerence(第七届国际药用菌大会(IMMC7)
北京
英文
769-780
2013-08-26（万方平台首次上网日期，不代表论文的发表时间）