Study on Apoptosis of Human promyelocytic leukemia HL-60 Cells Induced by Fucosterol via Death Receptor Pathway
(0) The purpose of this study is to investigate the effect of fucosterol on the induction ofapoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells.HL-60 Cells were treated with different concentrations of fucosterol at different time.MTT method was used to study fucosterol anti-tumor activity.Morphology observation was performed todetermine the effects of fucosterol on apoptosis of HL-60 cells.Flow cytometry (FCM) was used to detect the cell cycle.Laser scanning confocal microscope (LSCM) was used to analyze the expressions of Fas,FasL,Fadd and Caspase-8.Caspase activity kits were used to determine the activity of Caspase-8 and Caspase-3.The results showed fucosterol could inhibit the growth of HL-60 cells,and the apoptosis morphology for 48 h treatment was obvious,which showed cell protuberance,cytoplasm concentrated and apoptotic body.Fucosterol treatment for 24 h increased the protein expression of Fas,FasL,Fadd and Caspase-8.It also showed that the activity of Caspase-3 and Caspase-8 has increased significantly.In conclusion,Fucosterol could induce HL-60 cells apoptosis via death receptor pathway.
fucosterol Human promyelocytic leukemia apoptosis death receptor pathway
Yu-bin JI Dong-xue SONG Chen-feng JI
Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin University of C Center of Research on Life Science and Environmental Science,Harbin University of Commerce,Harbin 15
国际会议
西安
英文
1-4
2013-07-27（万方平台首次上网日期，不代表论文的发表时间）