Study on Apoptosis of Human promyelocytic leukemia HL-60 Cells Induced by Fucosterol via Mitochondrial Pathway
The purpose of this study is to investigate the effect of fucosterol on the induction ofapoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells.HL-60 Cells were treated with different concentrations of fucosterol at different time.MTT method was used to study fucosterol anti-tumor activity.Morphology observation was performed to determine the effects of fucosterol on apoptosis of HL-60 cells.Flow cytometry (FCM) was used to detect the cell cycle.Laser scanning confocal microscope (LSCM) was used to analyze mitochondrial membrane potential (MMP).Western blot was performed to analyze the expressions of Cyt-C,Caspase-9 and Caspase-3.The results showed fucosterol could inhibit the growth of HL-60 cells,and the apoptosis morphology for 48 h treatment was obvious,which showed cell protuberance,cytoplasm concentrated and apoptotic body.Fucosteroi treatment for 24 h decreased MMP in dose-dependent manners.It also induced the release of Cyt-C and the activation of Caspase-9 and-3.In conclusion,Fucosterol could induce HL-60 cells apoptosis through a mitochondrial pathway.
fucosterol Human promyelocytic leukemia apoptosis mitochondrial pathway
Chen-feng JI Ying LI Yu-bin JI
Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin University of C Center of Research on Life Science and Environmental Science,Harbin University of Commerce,Harbin 15
国际会议
西安
英文
5-8
2013-07-27（万方平台首次上网日期，不代表论文的发表时间）