The Role of Beclin1 Gene in Autophagy and Apoptosis Induced by Ionizing Radiation in MCF-7 Cells

摘要：

　　Objective To discuss the role of Beclinl gene in autophagy and apoptosis induced by ionizing radiation in MCF-7 cells.Methods MTT assay was used to detect the influence of cells proliferation when beclin-1 gene was over expression and interference.Flow cytometric analysis detected the change of MCF-7 cells apoptosis after irradiating by X-ray.Western blot method detected total protein changes ofbeclin-1.Results Beclin-1 gene was interferred partly,the amount of protein expression of MCF-7-beclinlRi cells reached to the lowest at 4 h,rose at 8 h,got to the most at 16 h and decreased at 32 h,but still held at higher level; Beclin-1 gene overexpression,the amount of protein expression increased gradually with the extension of time,and got to peak at 32 h.Conclusions Ionizing radiation can stimulate beclin-1 and exist relations of dose -effects in MCF-7 cells.

关键词： ionizing radiation autophagy apoptosis Beclin1

作者: QI Ya-li Liu Yan-jun ZHAO Da-li

作者单位: Department of Epidemiology,School of Public Health,Beihua University,Jilin 132001,China peoples Hospital of Chang chun city,Changchun 130021,China Jilin Entry-exit Inspection and Quarantine,Changchun 130021,China

会议类型: 国际会议

会议名称: 2013 International Conference on Advanced Engineering Materials and Architecture Sciences(2013先进工程材料与建筑科学国际会议)(ICAEMAS 2013)

会议地点: 西安

会议语种:英文

页码: 29-31

在线出版日期: 2013-07-27（万方平台首次上网日期，不代表论文的发表时间）