MOLECULAR MARKERS ENABLE THE IDENTIFICATION OF CONTAMINANTS IN PRODUCTION FIELDS OF ‘CAPTIVA’ ST.AUGUSTINEGRASS
To ensure the purity of a clonally propagated cultivar,strict guidelines are followed during its registration and release.Cultivar contamination can result from mechanical transfer of other cultivars/genotypes,seedlings,and random somatic mutation.Once contamination has occurred,it can be very difficult and time-consuming to coordinate an effective strategy to identify and contain the problem.‘Captiva’ is a recently released St.Augustinegrass Stenotaphrum secundatum (Walt.) Kuntze cultivar from the University of Florida.In mid-2009,reports began to surface in Florida of off-type plants with different leaf color,texture and growth habit from Captiva.In this study,eight AFLP primer combinations were used to identify genetic variants or contaminants in Captiva foundation,registered,and certified fields.Six other St.Augustinegrass cultivars were also included as checks.Genetic similarities between cultivars and putative contaminants were used to verify sample identity as well as associate off-types with cultivar checks.Forty-nine percent of the contaminants were identified as ‘Palmetto’,eight percent as ‘Raleigh’,seven percent as ‘Sapphire’,three percent as ‘Floratam’,one percent as ‘Floraverde’,and three percent of contaminants were not identified.UPGMA analysis and principal coordinate analysis confirmed these results.An AMOVA ran with only true Captiva samples indicated no significant variation among samples from different field types.This study provides further evidence that molecular markers can be successfully used to quickly identify contaminants and aid sod producers in efficiently quarantining problematic cultivar sources.
AFLPs fingerprinting St. Augustinegrass
Jennifer A. Kimball M. Carolina Zuleta Kevin E. Kenworthy Huanjung Lu Susana R. Milla-Lewis
Dept. ofCrop Science,N.C. State Univ.,Raleigh,NC 27695 USA Dept. of Agronomy,Univ. of Florida,Gainesville,FL 32611 USA University of Florida,Everglades Research and Education Center,Belle Glade,FL33430 USA
国际会议
The 12th International Turfgrass Research Conference(第十二届国际草坪学术大会)
北京
英文
267-273
2013-07-14（万方平台首次上网日期，不代表论文的发表时间）