SSR/CISP MARKER ANALYSIS FOR GENETIC RELATIONSHIP
Zoysia grasses are well adapted to warm climates and the transition zone in the United States.In this study,the genetic relationship of 11 turfgrass commercial cultivars and 12 experimental genotypes of Zoysia spp.was estimated using 24 Zoysia-specific simple sequence repeat (SSR) and six conserved-intron scanning primer (CISP) markers previously developed for the warm season grasses such as rice,sorghum,and Pennisetum.All 23 Zoysia accessions tested,except ‘Zorro’ and ‘Zeon’,were genetically fingerprinted using 51 allelic amplicons.The SSR markers were able to separate the cultivars ‘Empire’ and ‘El Toro’,which were previously inseparable using AFLP markers.The dendrogram constructed using the UPGMA method indicated that the accessions were classified into three clusters.All cultivars (or genotypes) in cluster I corresponded to cold tolerant individuals,while clusters II and III represented accessions with high or intermediate susceptibility to winter injury,respectively.Overall,accessions bred from either common parents or the same collection sites were categorized in the same cluster.These results demonstrate the effectiveness of Zoysia SSR markers and the transferability of six CISP markers for genetic diversity of these cultivars and experimental genotypes,providing a useful genetic tool for improvement of Zoysia germplasm.
CISP genetic similarity molecular marker SSR Zoysia
Loreto Araneda Jeonghwa Lee Zhenbang Chen Paul Raymer Geunhwa Jung
Stockbridge School of Agriculture,University of Massachusetts Amherst,MA 01003USA The National Academy of AgriculturalScience,Korea University of Georgia,USDA-ARS Dept. of Crop and Soil Sciences,University of Georgia
国际会议
The 12th International Turfgrass Research Conference(第十二届国际草坪学术大会)
北京
英文
299-303
2013-07-14（万方平台首次上网日期，不代表论文的发表时间）