HPLC DETERMINATION OF PHOTOSYNTHETIC PIGMENTS IN KENTUCKY BLUEGRASS WITH AN ENDCAPPED ODS-2 C18 COLUMN
The objective of this work was to select an effective method to evaluate plant photosynthetic pigment content and composition.Three modified high performance liquid chromatographic programs were tested to determine photosynthetic pigment composition with an endcapped Spherisorb ODS-2 C18 column.Program II outweighted program I in accordance with baseline profile and peak symmetry.Lutein was better eluted in program I,but program II resolved chlorophyll b and β-carotene better than program I.However,when photosynthetic pigments content was expressed relative to the content of chlorophyll b,no pronounced difference was observed in relative pigments content between program I and II.Program III showed very poor performance with the endcapped ODS-2 C18 column,and failed to completely resolve chlorophyll b.Program II was thereby suggested as an effective approach to analyze plant photosynthetic pigment content and composition.
endcapped spherisorb ODS-2 C18 column high performance liquid chromatography (HPLC) photosynthetic pigments relative pigment content shade
Xiaoling Sun Yuefei Xu He Zhou
Department of Horticulture,TianjinAgricultural University,Tianjin,300384,China College of Animal Science and Technology,Northwest A&FUniversity,Yangling,712100,China Departmentof Grassland Science,College of Animal Science andTechnology,China Agricultural University
国际会议
The 12th International Turfgrass Research Conference(第十二届国际草坪学术大会)
北京
英文
721-726
2013-07-14（万方平台首次上网日期，不代表论文的发表时间）