THREE-DIMENSIONAL OBSERVATION OF LEAF EPIDERMIS OF CREEPING BENTGRASS BY CONFOCAL LASER SCANNING MICROSCOPY

摘要：

　　The leaf epidermis of creeping bentgrass Agrostis stolonifera (L.) was observed with autofluorescence by confocal laser scanning microscopy (CLSM).The three-dimensional observation revealed that leaf epidermal cells in a layer were morphologically complicated,different in shape and thickness,adhered to each other like a jigsaw puzzle,and some stomata were slightly covered with adjacent cells.Leaf strings,fixed probably over veins,were remarkably observed to be fistulous,and “prickle hairs or trichomes were hollow.These observations suggest that the complex structures of creeping bentgrass leaf epidermis may relate to growth efficiency or possibly a survival strategy.To our knowledge,this is the first and preliminary report on three-dimensional observations of turfgrass leaf epidermis by CLSM.

关键词： autofluorescence CLSM leaf anatomy leaf string prickle hair stomata trichome turfgrass

作者: Masakazu J. Ushilo Shoko Kajiwara Taro Takemura Takashi Minowa

作者单位: Axxion Research Institute,Shintomi,Chuo-ku,Tokyo 104-0041,Japan Nanotechnology Innovation Station,National Institute for Materials Science. Sengen,Tsukuba,Ibaraki,3

会议类型: 国际会议

会议名称: The 12th International Turfgrass Research Conference(第十二届国际草坪学术大会)

会议地点: 北京

会议语种:英文

页码: 791-794

在线出版日期: 2013-07-14（万方平台首次上网日期，不代表论文的发表时间）