Isolation an antimicrobial action of endophytic fungi from sophora flavescens and effects on microorganism circumstances in soil
The aim of this study was to isolate the endophytic fungi from Sophora flavescens and define its antimicrobial action and structure.The effects of this active substance on soil microorganism circumstances were also conducted.Column chromatography and freeze drying were used to isolate and purify the antimicrobial substance.TLC biological autoradiography was applied to trace.HPLC method was employed to measure the purity.Analysis of the structure used 1H-NMR,13C-NMR and LC-MS methods.Plate method was applied to measure the effect on microorganism circumstances.The results showed that after isolating and purifying the fermentation liquor of BS001,the structure of bacteriostatic active composition was 6,7-(2E)dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-1,4-dione,which was identified by spectroscopy.The substance could increase the number of bacteria and fungi while decreased the number of antinomies in soil.A new antimicrobial substance 6,7-(2E)dibutenyl-5,8-dihydroxy-(Z)-cyclooct-2-ene-1,4-dione was extracted from fermenting liquor of BS001 which was an endophytic fungi of Sophora flavescens.It could promote the beneficial flora but detrimental flora.This conclusion provides exploiture foreground for biopharmaceuticals and biopesticide.
endophytic fungi biological activity soil microorganism circumstances Sophora flavescens
Lu He Na Liu Yong Wang Hongbo Xu Na Yu
College of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China The Information Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, Chin
国际会议
2013 International Symposium on Environmental Science and Technology(2013环境科学与技术国际会议)
大连
英文
208-212
2013-06-04（万方平台首次上网日期，不代表论文的发表时间）