Carboxylesterase from Spodoptera Litura:Immobilization and use for the degradation of pesticides

摘要：

　　The immobilization of carboxylesterase from Spodoptera Litura in mesoporus molecular sieves was studied in this paper.Two different types of mesoporous sieves (MCM-41,SBA-15) were initially used as enzyme support to compare the efficiency of immobilization.The outcome showed the maximum enzyme loadings,twice as many in MCM-41,are 0.0267 L/g in SBA-15 support.The most efficient pH value for immobilization was at 6.5 for both types of the support and the optimal immobilizing time was 2 h for SBA-15 and 4 h for MCM-41.Hence,the SBA-15 mesoporous support was determined as the enzyme immobilization matrix to continue the investigation because of its superior properties for enzyme immobilization.Then a series of characters of the free and immobilized Carboxylesterase were compared.As a result,the immobilized enzyme was substantiated to be obviously more stable than the free enzyme under the variant conditions.The experiments of pesticides degradation show that,relative to degradation in natural environment,immobilization enzyme had a higher ability to reduce the organic compounds with ester bonds.

关键词： Carboxylesterase Immobilization Mesoporous molecular sieves pesticide degradation

作者: Jianxiong Diao Guangyu Zhao Yuanqing Li Jinli Huang Ying Sun

作者单位: College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, Chin Department of Chemistry, China Agricultural University, Beijing 100193, China

会议类型: 国际会议

会议名称: 2013 International Symposium on Environmental Science and Technology(2013环境科学与技术国际会议)

会议地点: 大连

会议语种:英文

页码: 489-496

在线出版日期: 2013-06-04（万方平台首次上网日期，不代表论文的发表时间）