Apoptosis of Lymphocytes and Brain Tissue Cells Caused by Canine Distemper Virus

摘要：

　　To research apoptosis of lymphocytes and hrain tissue cells,this investigation was performed on 16 dogs infected by canine distemper virus and 6 health dogs as control.The lymphoid and brain samples were collected by the necoropsy.Then,the paraffin sections were made and stained by HE,and immunohistochemistry,that was anti-CDV sABC assay,and TUNEL assay.The results demonstrated that CDV had varying degree tropism to all lymphoid tissues and brain tissues.In lymphoid tissues,CDV antigen was detected in all lymphoid tissues of CDV infected dogs,especially in nucleus and cytoplasm of lymphocytes,follicular dendritic cells,interdigitating cells and macrophages.The apoptotic cells were associated with the infection of CDV.The cell in lymphoid tissues was infected badly and the apoptosis was developed evidently.In brain tissue,the most severe infectious cells were cerebral hlood vessel endothelia,ependymal cells,astrocytes and oligodendrocytes.Secondly it was microglias,neuron m nervenucleus.Less infectious cells were pyramidal cells and Purkinje cells.The most obvious apoptotic cells were cerebral blood vessel endothelia,astrocytes,and oligodendrocytes.Secondly it was ependymal cells and neurons in nervenucleus.As severe infection,more apoptotic cells were observed in the pyramidal cells,Purkinje cells and neurons in spinal cord.It follows that CDV has varying degree tropism to all lymphoid tissues and brain tissue.The stronger cells of tropism to CDV are,the faster and earlier apoptosis is.Apoptotic cells in lymphoid tissues and hrain tissues may be related to lymphocytopenia,immunosuppression and the appearance of diverse neurological signs in clinic.

关键词： apoptosis lymphoid tissue brain tissue immunosuppression neurological sign

作者: Pan Yaoqian Zhang Liuping Sun Yuqian Li Ruizhen Liu Zhike Yin Mei Tang Hairou Su Weiping Liu Xingyou

作者单位: College of Animal Science and Henan Higher Education Engineering Technology Research Center for Anim School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang,Jiangsu,212013,China Division of Neuroscience,Oregon Nationa Primate Research Centor,Oregon Health & Science University,5

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 189-193

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）