Isolation and Detection of Streptococcus gallolyticus subsp.gallolyticus from Organic Turkey Flocks
Streptococcus gallolyticus subsp.gallolyticus can be an opportunistic pathogen in animals and humans.lts role as a potential zoonotic pathogen is discussed.However,very little is known ahout the occurrence of this bacterium in turkey flocks.Thus we developed a selective isolation method for culturable S.gallolyticus suhsp.gallolyticus poultry faeces.First isolation tests were conducted with pigeon faeces from different holdings and the results showed that the tannase activity of S.gallohticus subsp.gallolyticus and its possibility to grow in the presence of sodium azide were successful characteristics to select the bacterium from streaks on solid media.Isolates were identified to species level by biochemical tests and to subspecies level by MALDI-TOF mass spectroscopy and sodA sequencing.Further lahoratory tests demonstrated that the application of the detection method is also useful for the isolation from turkey faeces.In a field study we investigated thirteen organic turkey flocks from ten different farms in Germany.Five fresh droppings were taken from each flock and analyzed within 24 h in the laboratory.Streptococcus gallolyticus subsp.gallolyticus occurred in all sampled flocks.The prevalence in positive droppings was 0.83.To the best of our knowledge,this is the first investigation that indicates a widely spread of S.gallolyticus subsp.gallolyticus in organic turkey flocks.The developed detection method seems to be a suitable tool to detect transmission ways and may contrihute to the investigation of the zoonotic potential of S.gallolyticus subsp.gallolyticus in future studies.
Schulz,J Hinse,D Dumke,J Dreier,J Habich,C Hartung,J
Institute for Animal Hygiene,Animal Welfare and Farm Animal Behaviour,University of Veterinary Medic Institut für Laboratoriums- und Transfusionsmedizin,Herz-und Diabeteszentrum Nordrhein-Westfalen,Uni
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
253-254
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）