The Effect of Sampling Methods on the Identification of Staphylococcus aureus in Cow Milk
Mastitis is one of the most common diseases of the dairy cows,and it is responsible for huge economic losses in the dairy industry.From the many causing agents,Staphylococcus aureus is the most prevalent mastitis pathogen in Hungary.In case of a massive Staphylococcus aureus infection in a dairy herd,a preventive program is the only reliable solution which means that large number of milk samples must be examined to identify the infected animals.In the practice the current method is culturing these samples on agar plates.In our first experiment we examined how the long-term congelation can alter the microbiological results.We could culture the Staphylococcus aureus bacterium after one year,so the bacterium definitely can survive that maximum 1-1.5 months what it spends in the farms deep freezer before the veterinarian sends it to the laboratory.On the other hand we also examined how the sampling procedure can affect the results.In two experiments,the effectiveness of pre-and post-milking composite samples,and individual quarter samples were compared.It is generally believed that post-milking samples are more reliable than the pre-milking ones,but it has turned out in both experiments,that the pre-milking composite milk samples are more effective to identify the infected animals than after-milking composite samples.We also found that culturing the same sample 3 times raises the accuracy while the examination of individual quarter samples cost four times more than composite samples and the results was only slightly better,so this method is unprofitable and not advised in practice.
Kovacs,P Szita,G Jurkovich,V Konyves,L Solymosi,N Brydl,E
Szent Istvan University Faculty of Veterinary Science Dep.of Animal Hygiene,Hungary Szent Istvan University Faculty of Veterinary Science Dep.of Food Hygiene,Hungary
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
334-335
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）