Exploring Novel Therapeutics for Cryptosporidium:Identification and in vitro Screening of Phylomer(R)Peptides and Chinese Herbs
Cryptosporidium is a protozoan pathogen that causes prolonged diarrhea in humans,livestock,wild animals,birds,fish and reptiles.The parasite can be transmitted via the faecal-oral route and by contaminated water.The only approved treatment in humans,nitazoxanide (NTZ ),is ineffective in immunocompromised individuals and new anti-cryptosporidial therapeutic agents are urgently required.Cryptosporidium lacks de novo purine synthesis,and is exclusively dependant on purine salvage from its host.Inhibition of inosine 5 monophosphate dehydrogenase (IMPDH),a purine salvage enzyme that is essential for DNA synthesis,offers a potential drug target against this parasite.A previous study used a yeast-two-hybrid system to identify phylomer peptides (constructed from the genomes of phylogenetically diverse bacteria)that targeted the IMPDH of C.parvum (IMPDHcp).Here we present dost response analysis of two phylomers,which exhibited significant growth inhibition (81.2%-83.8% inhibition; P<0.05),when screened against C.parvum in vitro.We also present data on the anticryptosporidial activity and cytotoxicity of 7 traditional Chinese medicines (TCMs)in vitro.
Yang,R Armson,A Ryan,U.M Jefferies,R Woh,C.K Weldt,T Estcourt.A Armstrong,T Hopkins,R Watt,P Reid,S
School of Veterinary and Life Sciences,Murdoch University,Murdoch,Australia School of Veterinary and Life Sciences,Murdoch University,Murdoch,Australia;Phylogica,Telethon Insti Phylogica,Telethon Institute for Child Health Research,Subiaco,Australia
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
365-368
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）