Ecotoxic Effects of a Veterinary Food Additive,CuSO4 0n Antioxidant Enzymes and mRNA Expressions in Earthworms
This study was aimed to investigate the effect of veterinary food additive CuS04 (CuS04)at different concentrations and different early exposure phases,on the ecotoxic responses of the earthworms Eisenia fetida (E.fetida).Earthworms were exposed to increasing concentrations of CuSO4 (50,100,200 and 400 mg/kg dry soil by Cu)and for different periods of time (5,10 and 15 days).The following biomarkers were measured: Catalase (CAT),superoxide dismutase (SOD),glutathione peroxides (GPx),glutathione-S-transferase (GST )activity and malonaldehyde (MDA)concentration.Moreover,gene expression analysis was also conducted as molecular biomarkers: the expression of metallothioneins (MT)gene and heat shock protein 70 (Hsp70)gene.These biomarker responses varied in relation to concentrations and exposure times.The results showed antioxidant enzymes had the efficiency to provide antioxidant defenses against the stressor except GST and highlighted expression of MT and Hsp70 could be used as reliable and sensitive molecular tools with predictive possibilities to monitor the ecotoxicity of veterinary feed additive CuS04.
veterinary food additive antioxidant expression CuSO4 ecotoxicity
Xiong Wenguang Bai Ling Muhammad Rizwan-UI-Haq Chen Chao Sun Yongxue
National Laboratory of Safety Evaluation(Environmental Assessment)of Veterinary Drugs,South China Agricultural University,Guangzhou,China
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
375-375
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）