Both Flagella and F4 Fimbriae Contribute F4ac+Enterotoxigenic Escherichia coli Adhering to IPEC-J2 Cells in vitro

摘要：

　　While it is known that bacterial tlagella agella generally contribute to pathogen virulence,the role of flagella in the pathogenesis of F4ac + Enterotoxigenic Escherichia coli (ETEC)mediated neonatal and post-weaning diarrhea (PWD)is not currently understood.We targeted the reference C83902 ETEC strain (08∶ H19∶ F4ac + LT + STa + STb + ),to construct isogenic mutants in the fliC,motA,and faeG genes.Both the AfliC and AfaeG mutants had a reduced ability to adhere to porcine intestinal epithelial IPEC-J2 cells.F4 fimbriae expression was significantly down-regulated after deletingf/iC,which revealed that co-regulation exists between flagella and F4 fimbriae.However,there was no difference in adhesion between the AmotA mutant and its parent strain.These data demonstrate that both flagella and F4 fimbriae are required for efficient F4ac + ETEC adhesion in vitro.

关键词： flagella F4 fimbriae adherence

作者: Zhou Mingxu Duan Qiangde Zhu Xiaofang Guo Zhiyan Li Yinchau Philip R.Hardwidge Zhu Guoqiang

作者单位: College of Veterinary Medicine,Yangzhou University,China Department of Dermatology of Clinical Medical School,Yangzhou University,China College of Veterinary Medicine,Kansas State University,Manhattan,USA

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 480-482

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）