Monomeric Flagellin May be More Suitable as an Adjuvant Than Polymeric Flagellin

摘要：

　　This paper describes the biological differences between monomeric and polymeric flagellin.Bacterial flagellin,an effective immune adjuvant,can be expressed as a monomer or polymer depending on the folding and assembly process.However,the biological differences between monomeric and polymeric flagellin are unclear.Here,we prepared the monomer (mfliC/M )and polymer chimeric flagellin (pfliC/M)containing the ectodomain of the matrix 2 protein of influenza A virus in the hypervariable region of fliC flagellin to compare their biological efficacy.The results indicated that they showed distinct circular dichroism spectra and that the polymerization of mfliC/M was ruuch lower than for pfliC/M.Three hours after transfection into macrophages,mfliC/M could induce more higher interleukin-1β secretion.Serum antibody assay indicated that pfliC/M could induce significantly higher flagellin-specific antibody production two weeks after immunization compared with mfliC/M.Thus,the folding and assembly processes of flagellin might contribute to their difference and monomeric flagellin may be more suitable as an adjuvant than polymeric flagellin.

作者: Hu Maozhi Pan Zhiming Yang Yun Meng Chuang Jiao Xinan

作者单位: Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou 225009,China Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou 225009,China;Ministry of Education K

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 505-509

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）