The Role of Hsp90αin Apoptosis and Damages of Primary Myocardial Cell Cultures of Neonatal Rats after Heat Stress
This study describes the relation among the heat induced pathological changes and apoptosis,and the variations of protective Hsp90α and its corresponding mRNA in the heat stressed primary myocardial cells of neonatal rats in vitro.Levels of enzyme CK increased from the beginning of heat stress and acute cellular lesions characterized by vacuolar degeneration and necrosis in the heat stressed cells were observed after 40 min of heat stress,suggesting that the myocardial cells in vitro were obviously damaged by higher temperature.The levels of cleaved Caspase-3 and Cytochrome C which concerned to apoptosis increased significantly after 40 min of heat stress while Hsp90α protein significantly decreased,and the levels of cleaved Caspase-3 and Cytochrome C decreased while Hsp90α significantly increased after 6 h of heat stress,indicating that early depletion of Hsp90α coincides with a high rate of necrosis and apoptosis in heat stressed myocardial cells while in surviving cells Hsp90α increases after 6 h of heat stress again with significantly less apoptosis.
Ali I Hartung J Bao E Lv Y Adam A Buriro R Liu Z Zhang M Tang S Cheng Y Chen H
College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China Institute for Animal Hygiene,Animal Welfare and Farm Animal Behaviour,University of Veterinary Medic College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;College of Ainma College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;College of Anima
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
510-512
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）