Hsp70 and HSF-1 Expression Is Altered in the Tissues of Pigs Transported for Various Periods of Times
The aim of this study was to assess changes of Hsp70 and HSF-I protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time.Twenty pigs were randomly divided into four groups (0 h,1 h,2 h,and 4 h of transportation).A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation.Histopathological changes in the heart,liver,and stomach indicated that these organs sustained different degrees of injury.Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (P<0.05 )in the stomach.hsp70 mRNA levels decreased significantly (P<0.05)in the heart after 4 h of transportation.However,mRNA expression increased significantly in the liver after 1 (P<0.05 )and 4 h (P<0.01)of transportation,and increased significantly in the stomach of the transported pigs after 1,4 (P<0.01),and 2h(P<0.05).HSF-1 levels were reduced at 1 and 4 h (P<0.05)only in the hearts of transported pigs.These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation.
heat shock factor-1 heat shock protein 70 pig transport stress-sensitive organs transport time
Zhang Miao Yue Zhenhua Liu Zhijun Ali Islam Buriro Rehana Tang Shu Bao Endong Hartung J(o)rg
College of Animal Science and Technology,Jinling Institute of Technology,Nanjing 210038,China;Colleg College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China Institute for Animal Hygiene,Animal Welfare and Farm Animal Behaviour,University of Veterinary Medic
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
525-527
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）