Construction and Characterization of cya,crp Deletion Mutants from E.coli Causing Goose Salpingitis and Peritonitis

摘要：

　　Goose salpingitis and peritonitis are fatal disease caused by Avian pathogenic Escherichia coli (APEC)that usually occurs in adult goose especially female goose at laying period,because of the high mortality and morbidity accompanied with low fertility and hatching rate.It has become one of the most important diseases in the goose industry in China.To date,there is still no commercial vaccine for controlling this disease.In order to improve new way for creating effective attenuated live vaccine candidate against APEC,this study constructed APEC G8107 (02∶K89)cya and crp gene mutant strains G8109 (G8107 AcVa)and G8111 (G8107 AcyaAcrp)which has no antibiotic resistant marker by the λ Red-based recombination system,and then compared bio-characteristics and virulence among the mutants and the parent strain.

关键词： APEC cya gene crp gene mutant strains bio-characteristics virulence assays

作者: Zhang Zhiqiang Zhu Chunhong Meng Xia Hou Huayan Wang Kai Jiang Lu Li Yonghui Zhu Guoqiang

作者单位: College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China Jiangsu Institute of Poultry Science,Yangzhou 225125,China

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 540-540

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）