Nanoparticulated Heat-Stable(STa)and Heat-Labile B Subunit(LTB)Recombinant Toxin Improves Vaccine Protection Against Enterotoxigenic Escherichia coli Challenge in Mouse

摘要：

　　Enterotoxigenic Escherichia coli (ETEC)remains a major cause of diarrheic disease in developing areas,for which there is no effective vaccine available.In this study,we genetically engineered a recombinant heat-stable enterotoxin (STa)coupled to the subunit B of heat-labile enterotoxin (LTB ).This fusion protein,STa-LTB,possesses a single amino acid substitution at position 14 of STa.Our data demonstrates that the enterotoxicity of STa in STa-LTB was dramatically reduced.A gelatin nanovaccine candidate was prepared using the purified STa-LTB fusion protein characterized with an entrapment efficiency of 84.88%±6.37% and smooth spheres size ranges of 80e200 nm.

作者: Deng Guangcun Zeng Jin Jian Minjie Liu Wenmiao Zhang Zhong Liu Xiaoming Wang Yujiong

作者单位: College of Life Science,Ningxia University

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 541-541

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）