Reduction of Infectious Bursal Disease Virus Replication by shRNAs Targeting the VP1 and VP2 Genes Driven by Chicken U6 Promoter

摘要：

　　Infectious bursal disease virus (IBDV)causes a highly contagious and immunosuppressive disease in young chickens and results in considerable economic losses for the poultry industry.To suppress the replication of IBDV.two short hairpin RNAs (shRNAs)were designed for targeting the VPI and VP2 genes of IBDV.Recombinant plasmids carrying each shRNA or two shRNAs were constructed based on vector pSilencer2.1-U6 in which the human U6 promoter was replaced with chicken U6 promoter.In chicken embryo fibroblasts,transfection with these shRNA plasmids 24 h before infection with IBDV B87 reduced 50% tissue culture infectious doses (TCID50)from by 108.75 TCID50/0.1 mL to 103.75-101.0 TCID50/0.1 mL.

作者: Ouyang Wei Ma Jinrong Wang Yongqiang Qin Liting Jiang Jieyuan Fan Hongjie Wang Xiaomei Wang Yongshan

作者单位: Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture;Institute State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academ Department of Microbiology and Immunology,College of Veterinary Medicine,Nanjing Agricultural Univer

会议类型: 国际会议

会议名称: XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))

会议地点: 南京

会议语种:英文

页码: 542-542

在线出版日期: 2013-05-05（万方平台首次上网日期，不代表论文的发表时间）