Local Immune Response of Ascarid Invasion Affected Intestine on Different Food Supplement in Chicken
In agriculture nourishment of breeding birds plays a key role in birds health and development of their immune system,therefore affecting quality and taste of products for consumers.The aim of this study was to evaluate the local tissue immune responses in broilers small intestine after different food additives,focusing on the imruunohistochemical detection of the cytokines and antimicrobial proteins.Materials and methods.We analysed the small intestine of 7,28 and 42 days old 40 broilers receiving Jerusalem artichoke root powder in different combinations with Lactobacillus reuteri and Perliococcus; 14 chicken were used for controls.The small intestine of the birds were routinely stained and analyzed also for IL-IO and antimicrobial protein beta-defensin 2.Results revealed dramatically destructed villi as well as local prominent inflammation in intestine of all groups including the control.Moreover.massive ascarides eggs invasion was discovered in the lymphatic and blood vessels of the intestinal wall.The Jerusalem artichoke root powder in the 0.5% concentration decreased IL-IO producing cells on the 28th day and increased them on the 42nd day.In the L.reuteri group number of IL-IO producing cells decreased after 0.5% and increased after 3% Jerusalem artichoke root powder addition in food.Food and Pediococcus with Jerusalem artichoke root powder on the 28th day induced decrease of IL-IO producing cells,but on the 42nd day increase of them.Conclusions.Changes in broilers small intestine local immune defence seems strongly depend on the invasion of the ascarides.On the 28th day 0.5% Jerusalem artichoke root powder decreases IL-IO expression indicating the immunity inhibitory nature of this concentration powder.The local immunity response increases again at the 42 day possibly indicating both combined Jerusalem artichoke powder and bacteria positive influence and also ascaridosis stimulation of the local immunity in intestine of chicken.
Pilmane M Valdovska A Pereca J Konrade L Zitare I Jemeljanovs A
Institute of Anatomy and Anthropology,Riga Stradins University,Latvia Research Institute of Biotechnology and Veterinary Medicine Sigra of the Latvia University of Agri
国际会议
XVIth International Congress on Animal Hygiene(第十六届动物卫生国际会议(ISAH 2013))
南京
英文
559-561
2013-05-05（万方平台首次上网日期，不代表论文的发表时间）