Studies on the Immobilized-Lipase-Catalyzed Esterification of Alkyl Oleates in Solvent-free Systems
The esterification of oleic acid with alkyl alcohols in solvent-free systems was catalyzed by an immobilized lipase from Candida sp.99-125.The influence of several factors,including enzyme concentration,temperature,molar ratio between oleic acid and alkyl alcohols,and structure of alcohol was also investigated.The results indicated that the reactions catalyzed by lipase at 20 ℃,in the presence of 3% (w/w) lipase,on the molar ratio of 1∶1 between oleic acid and alcohols,afforded products in high yield.It showed high selectivity to primary and low selectivity to secondary alcohols and tertiary alcohols because of the sterically hindered effect.Methanol has certain toxicity on the activity of the lipase.The lipase from Candida sp.99-125 was identified to be an effective catalyst in the esterification of alcohol and oleic acid at low temperature.
Enzymatic catalysis Immobilized Lipase Esterification Oleates
Hui Zhong Zheng Fang Baohua Zou Xin Li Kai Guo
School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing, PR China College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology,Nanjing, P
国际会议
太原
英文
19-23
2013-01-13（万方平台首次上网日期，不代表论文的发表时间）