A new resonance Rayleigh scattering assay for the determination of trace Hg(Ⅱ) using the immunonanogold as probe
Nanogold (NG) in size of 10 nm was prepared by the NaBH4 procedure.A new ligand 6-mercaptonicotinic acid (MNA) was used to couple both methyhnercury chloride (CH3HgCl) and carrier protein to obtain an immunogen,it was immunized BALB/C mice,and the spleen cells of immunized mice were fused with myeloma cells.The monoclonal antibody (mAb) against mercury (Ⅱ) ions was produced by the hybridoma technique.The mAb was labeled the NG to prepare an ilnmunonanogold (ING) probe for Hg(Ⅱ).In pH 5.4 Na2HPO4-citric acid buffer solution and under the condition of ultrasonic irradiation,the ING particles were aggregated un-specifically to form big particles that exhibited a strong resonance Rayleigh scattering (RRS) peak at 580 nm.When the Hg(Ⅱ) was added,the specific immunoreaction of ING-Hg(Ⅱ)take place,and the ING-Hg(Ⅱ) irnmunocomplex dispersed in the solution that caused the RRS intensity decreasing linearly at 580 nm.The decreased intensity was linear to Hg(Ⅱ) concentration in the range of0.025-10 μmol/L,with a detection limit of 1.1 nmol/L Hg(Ⅱ).
Hg(Ⅱ) 6-mercaptonicotinic acid monoclonal antibody immunonanogold resonance Rayleigh scattering spectral probe
Lili Xu Zhiliang Jiang Yuzhen Wang Hong Yang Anping Deng
School of Environment and Resource, Guangxi Normal University, Guilin 541004, China College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123,
国际会议
秦皇岛
英文
221-224
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）